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iscript advanced cdna synthesis kit  (Bio-Rad)


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    Bio-Rad iscript advanced cdna synthesis kit
    Expression of Sir2-regulated genes was higher in cells grown in low nicotinic acid. a) RNA was isolated from wild-type cells (LRY2992) grown in varying concentrations of nicotinic acid. The RNA was used to make <t>cDNA,</t> which was analyzed by qPCR to quantify the TNA1 gene normalized to CWC15, which is not regulated by Sir2. Bars represent the average and standard deviation of RNA from 4 independent cultures. b) RNA was isolated and analyzed as in A using wild-type (LRY2992) or sir2Δ (LRY2993) cells grown in the highest and lowest concentrations of nicotinic acid. c) The same wild-type cDNA samples used in a were analyzed using primers amplifying gene F22319g. d) The same cDNA samples used in b were analyzed using primers amplifying gene F22319g. Significance was determined using an unpaired 2-tailed t-test. NS, not significant; * P < 0.05; ** P < 0.01.
    Iscript Advanced Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48063 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iscript advanced cdna synthesis kit/product/Bio-Rad
    Average 99 stars, based on 48063 article reviews
    iscript advanced cdna synthesis kit - by Bioz Stars, 2026-04
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    1) Product Images from "The deacetylase Sir2 is the primary sensor driving transcriptional changes in response to low NAD + in the yeast Kluyveromyces lactis"

    Article Title: The deacetylase Sir2 is the primary sensor driving transcriptional changes in response to low NAD + in the yeast Kluyveromyces lactis

    Journal: Genetics

    doi: 10.1093/genetics/iyag025

    Expression of Sir2-regulated genes was higher in cells grown in low nicotinic acid. a) RNA was isolated from wild-type cells (LRY2992) grown in varying concentrations of nicotinic acid. The RNA was used to make cDNA, which was analyzed by qPCR to quantify the TNA1 gene normalized to CWC15, which is not regulated by Sir2. Bars represent the average and standard deviation of RNA from 4 independent cultures. b) RNA was isolated and analyzed as in A using wild-type (LRY2992) or sir2Δ (LRY2993) cells grown in the highest and lowest concentrations of nicotinic acid. c) The same wild-type cDNA samples used in a were analyzed using primers amplifying gene F22319g. d) The same cDNA samples used in b were analyzed using primers amplifying gene F22319g. Significance was determined using an unpaired 2-tailed t-test. NS, not significant; * P < 0.05; ** P < 0.01.
    Figure Legend Snippet: Expression of Sir2-regulated genes was higher in cells grown in low nicotinic acid. a) RNA was isolated from wild-type cells (LRY2992) grown in varying concentrations of nicotinic acid. The RNA was used to make cDNA, which was analyzed by qPCR to quantify the TNA1 gene normalized to CWC15, which is not regulated by Sir2. Bars represent the average and standard deviation of RNA from 4 independent cultures. b) RNA was isolated and analyzed as in A using wild-type (LRY2992) or sir2Δ (LRY2993) cells grown in the highest and lowest concentrations of nicotinic acid. c) The same wild-type cDNA samples used in a were analyzed using primers amplifying gene F22319g. d) The same cDNA samples used in b were analyzed using primers amplifying gene F22319g. Significance was determined using an unpaired 2-tailed t-test. NS, not significant; * P < 0.05; ** P < 0.01.

    Techniques Used: Expressing, Isolation, Standard Deviation



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    Expression of Sir2-regulated genes was higher in cells grown in low nicotinic acid. a) RNA was isolated from wild-type cells (LRY2992) grown in varying concentrations of nicotinic acid. The RNA was used to make cDNA, which was analyzed by qPCR to quantify the TNA1 gene normalized to CWC15, which is not regulated by Sir2. Bars represent the average and standard deviation of RNA from 4 independent cultures. b) RNA was isolated and analyzed as in A using wild-type (LRY2992) or sir2Δ (LRY2993) cells grown in the highest and lowest concentrations of nicotinic acid. c) The same wild-type cDNA samples used in a were analyzed using primers amplifying gene F22319g. d) The same cDNA samples used in b were analyzed using primers amplifying gene F22319g. Significance was determined using an unpaired 2-tailed t-test. NS, not significant; * P < 0.05; ** P < 0.01.

    Journal: Genetics

    Article Title: The deacetylase Sir2 is the primary sensor driving transcriptional changes in response to low NAD + in the yeast Kluyveromyces lactis

    doi: 10.1093/genetics/iyag025

    Figure Lengend Snippet: Expression of Sir2-regulated genes was higher in cells grown in low nicotinic acid. a) RNA was isolated from wild-type cells (LRY2992) grown in varying concentrations of nicotinic acid. The RNA was used to make cDNA, which was analyzed by qPCR to quantify the TNA1 gene normalized to CWC15, which is not regulated by Sir2. Bars represent the average and standard deviation of RNA from 4 independent cultures. b) RNA was isolated and analyzed as in A using wild-type (LRY2992) or sir2Δ (LRY2993) cells grown in the highest and lowest concentrations of nicotinic acid. c) The same wild-type cDNA samples used in a were analyzed using primers amplifying gene F22319g. d) The same cDNA samples used in b were analyzed using primers amplifying gene F22319g. Significance was determined using an unpaired 2-tailed t-test. NS, not significant; * P < 0.05; ** P < 0.01.

    Article Snippet: DNA was removed from RNA using DNaseI (New England Biolabs M0303S) according to the manufacturer's instructions. cDNA was generated from 2 μg RNA using the iScript Advanced cDNA Synthesis Kit (Bio-Rad 1725038) according to the manufacturer instructions.

    Techniques: Expressing, Isolation, Standard Deviation